Vitamin A (VA) BioAssay ELISA Kit (Rat)-Vitamin A (VA) BioAssay ELISA Kit (Rat)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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產(chǎn)品描述: Sample Type:Serum, plasmaIntended Use:For the quantitative determination of rat vitamin A (VA) concentrations in serum and plasma.Sensitivity:233.598pg/mlRange:312.5-5000pg/mlSpecificity:This assay has high sensitivity and excellent specificity for detection of rat VA. No significant cross-reactivity or interference between rat VA and analogues was observed.Test Principle:This BioAssay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated VA. The competitive inhibition reaction is launched between with HRP-conjugated VA and VA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of VA in the sample. The color development is stopped and the intensity of the color is measured.Kit Components:Assay plate, 1x96 wellsStandard (10X), 1x200ulHRP-conjugate (100X), 1x60ulHRP-conjugate Diluent, 1x10mlSample Diluent, 2x20mlWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10mlAdhesive Strip (For 96 wells), 4x stripsInstruction manualStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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