Cysteine Rich Secretory Protein 3 (CRISP3), BioAssay ELISA Kit (Human)-Cysteine Rich Secretory Protein 3 (CRISP3), BioAssay ELISA Kit (Human)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Cysteine Rich Secretory Protein 3 (CRISP3), BioAssay ELISA Kit (Human)

英文名字: Cysteine Rich Secretory Protein 3 (CRISP3), BioAssay ELISA Kit (Human)

供應商: USBiological

產(chǎn)品貨號: 352815

產(chǎn)品報價: ￥1/96 Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.Sample Type:Cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and tissue homogenatesIntended Use:For quantitative detection of human CRISP3 in cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and tissue homogenates.Sensitivity:<50pg/mlRange:1.56-100ng/mlSpecificity:Natural and recombinant human CRISP3Test Principle:This human CRISP3 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been precoated onto 96-well plates. Standards(NSO, N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CRISP3 amount of sample captured in plate.Kit Components:96-well plate precoated with human CRISP3 antibody, 1ST0000-100, Lyophilized recombinant human CRISP3 standard, 100ng/tube×2Biotinylated human CRISP3 antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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保存建議: 建議收到Cysteine Rich Secretory Protein 3 (CRISP3), BioAssay ELISA Kit (Human)產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。