EPO (Erythropoietin), BioAssay ELISA Kit (Rat)-EPO (Erythropoietin), BioAssay ELISA Kit (Rat)代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: EPO (Erythropoietin), BioAssay ELISA Kit (Rat)

英文名字: EPO (Erythropoietin), BioAssay ELISA Kit (Rat)

供應(yīng)商: USBiological

產(chǎn)品貨號: 352844

產(chǎn)品報價: ￥1/96 Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193aa propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process.Sample Type:Cell culture supernates, serum, plasma (heparin) and tissue homogenatesIntended Use:For quantitative detection of rat EPO in cell culture supernates, serum, plasma(heparin) and tissue homogenates.Sensitivity:<10pg/mlRange:46.9-3000pg/mlSpecificity:Natural and recombinant rat EPOTest Principle:This rat EPO BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EPO has been precoated onto 96-well plates. Standards(E.coli, T44-K226) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat EPO amount of sample captured in plate.Kit Components:96-well plate precoated with rat EPO antibody, 1Lyophilized recombinant rat EPO standard, 10ng/tube×2Biotinylated rat EPO antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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保存建議: 建議收到EPO (Erythropoietin), BioAssay ELISA Kit (Rat)產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。