- 中文名稱
Kallikrein-6, BioAssay ELISA Kit (Human)
- 英文名字
- Kallikrein-6, BioAssay ELISA Kit (Human)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號
- 352954
- 產(chǎn)品報價
- ¥1/96 Tests

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- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金,在線支付寶及網(wǎng)銀支付,或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞

- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理,是行業(yè)中少有的致力于服務(wù)客戶,幫助客戶,且擁有獨(dú)立的專業(yè)銷售團(tuán)隊、技術(shù)支持團(tuán)隊、市場營銷團(tuán)隊、進(jìn)出口報關(guān)團(tuán)隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng),專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù),在意您的成功!
- 產(chǎn)品描述
- KLK6(Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.Sample Type:Cell culture supernates, cell lysates, serum, plasma (heparin, EDTA) and tissue homogenatesIntended Use:For quantitative detection of human Kallikrein-6 in cell culture supernates, cell lysates, serum, plasma (heparin, EDTA) and tissue homogenates.Sensitivity:<3pg/mlRange:78-5000pg/mlSpecificity:Natural and recombinant human Kallikrein-6Test Principle:This human Kallikrein-6 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein-6 has been precoated onto 96-well plates. Standards(NSO) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallikrein-6 amount of sample captured in plate.Kit Components:96-well plate precoated with human Kallikrein-6 antibody, 1Lyophilized recombinant human Kallikrein-6 standard, 10ng/tube×2Biotinylated human Kallikrein-6 antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產(chǎn)品特點(diǎn)
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- 保存建議
- 建議收到Kallikrein-6, BioAssay ELISA Kit (Human)產(chǎn)品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶提供了諸多超值選擇。
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