Relaxin 3, BioAssay ELISA Kit (Human)-Relaxin 3, BioAssay ELISA Kit (Human)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Relaxin 3, BioAssay ELISA Kit (Human)

英文名字: Relaxin 3, BioAssay ELISA Kit (Human)

供應(yīng)商: USBiological

產(chǎn)品貨號: 353019

產(chǎn)品報價: ￥1/96 Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: Relaxin-3 is a neuropeptide which is highly conserved in species ranging from flies, fish, rodents and humans. It is a member and ancestral gene of the relaxin family of peptides, which includes the namesake hormone relaxin (designated 'H2 relaxin' in humans) which mediates peripheral actions during pregnancy and which was found to relax the pelvic ligament in guinea pigs almost a century ago. The broad distribution of relaxin-3 fibres/RXFP3 within several key neuronal circuits suggests an ability to modulate a broad range of behaviours. This has been confirmed in a growing number of rodent studies, which demonstrate relaxin-3 is able to modulate arousal, the response to stress, feeding/metabolism and memory; and likely plays a role in the generation/regulation of hippocampal theta rhythm.Sample Type:Cell culture supernates, serum and plasma (heparin)Intended Use:For quantitative detection of human Relaxin 3 in cell culture supernates, serum and plasma( heparin).Sensitivity:<10pg/mlRange:31.2-2000pg/mlSpecificity:Natural and recombinant human Relaxin 3Test Principle:This human Relaxin 3 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Relaxin 3 has been precoated onto 96-well plates. Standards(E. coli, D119-C142 (A) & A27-R53(B)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Relaxin 3 amount of sample captured in plate.Kit Components:96-well plate precoated with human Relaxin 3 antibody, 1Lyophilized recombinant human Relaxin 3 standard, 10ng/tube×2Biotinylated human Relaxin 3 antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產(chǎn)品特點: 針對Relaxin 3, BioAssay ELISA Kit (Human)該產(chǎn)品的特點優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。

保存建議: 建議收到Relaxin 3, BioAssay ELISA Kit (Human)產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。