The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components,including collagen,gelatin,fibronectin,laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester,lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-13 (also designated collagenase-3) is produced by breast carcinomas and degrades collagen type I,II,III.
應(yīng)用類型
Western blotting (0.5-4 ug/ml), immunoprecipitation and Immunohistochemistry (works with paraffin imbedded sections). However, the optimal concentrations should be determined individually. The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 454 of rat MMP-13