- 中文名稱
Endostatin (ES) BioAssay ELISA Kit (Rat)
- 英文名字
- Endostatin (ES) BioAssay ELISA Kit (Rat)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- 353399
- 產(chǎn)品報(bào)價(jià)
- ￥1/48Tests ￥1/96Tests
- 產(chǎn)品說(shuō)明書
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- 購(gòu)買方式
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- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- The Rat Endostatin (ES) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of ES in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.Detection Range:1.23 - 100ng/mlSensitivity:<0.54ng/mlPrecision:Intra-Assay: CV<10%Inter-Assay: CV<12%Test Principle:This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for ES has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled ES and unlabeled ES (standards or samples) for limited binding sites on the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of ES in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of ES in the sample.Kit Components:*353399A: Microtiter Plate, 96 wells, Pre-coated, ready to use.*353399B: Standard, 2x1vial 353399C: Standard Diluent, 1x20ml*353399D: Detection Reagent A, 1x120ul *353399E: Detection Reagent B, 1x120ul 353399F: Assay Diluent A, 1x12ml353399G: Assay Diluent B, 1x12ml353399H: TMB Substrate, 1x9ml353399K: Stop Solution, 1x6ml353399L: Wash Buffer, 30X, 1x20mlPrecaution:The Stop Solution (353399K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *353399A, *353399B, *353399D and *353399E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Materials Required But Not Supplied:1. Microplate reader with 450 ± 10nm filter.2. Precision single or multi-channel pipettes and disposable tips.3. Eppendorf Tubes for diluting samples.4. Deionized or distilled water.5. Absorbent paper for blotting the microtiter plate.6. Container for Wash SolutionSample Preparation and Storage:Serum:Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000xg. Assay freshly prepared serum immediately or store samples in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Plasma:Collect plasma using EDTA or heparin as anticoagulant. Centrifuge samples for 15 minutes at 1000xg at 2-8° within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Tissue Homogenates:The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed thoroughly in ice-cold PBS (0.02M pH 7.0-7.2) to remove excess blood and weighed before homogenization. The tissues were minced to small pieces and homogenized in 5-10ml of PBS with a glass homogenizer on ice (Micro Tissue Grinders works too). The resulting suspension was sonicated with an ultrasonic cell disrupter or subjected to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifuged for 5 minutes at 5000×g. The supernatant was removed and assayed immediately or aliquoted and stored at -20°C or lower.Cell Lysates: Cells must be lysed before assaying according to the following directions. 1. Adherent cells should be detached with trypsin and then collected by centrifugation (suspension cells can be collected by centrifugation directly). 2. Wash cells three times in cold PBS. 3. Resuspend cells in PBS (1×) and subject the cells to ultrasonication 4X (or freeze cells at -20°C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle 3X.) 4. Centrifuge at 1500×g for 10 minutes at 2-8°C to remove cellular debris.Cell Culture Supernatants and Other Biological Fluids:Centrifuge samples for 20 minutes at 1000×g. Remove particulates and assay immediately or store samples in aliquots at -20°C or -80°C. Avoid repeated freeze/thaw cycles.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
- 產(chǎn)品特點(diǎn)
- 針對(duì)Endostatin (ES) BioAssay ELISA Kit (Rat)該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說(shuō)明書。
- 保存建議
- 建議收到Endostatin (ES) BioAssay ELISA Kit (Rat)產(chǎn)品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說(shuō)明書為準(zhǔn)。下單前，請(qǐng)瀏覽說(shuō)明書確認(rèn)。
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