- 中文名稱
BAFF (B-Cell Activating Factor) BioAssay ELISA Kit (Human)
- 英文名字
- BAFF (B-Cell Activating Factor) BioAssay ELISA Kit (Human)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- 354179
- 產(chǎn)品報(bào)價(jià)
- ￥1/96Tests
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- The BAFF (B-Cell Activating Factor) BioAssay ELISA Kit (Human) is a quantitative sandwich assay for the detection of BAFF in human serum, plasma, tissue homogenates and other biological fluids.Detection Range:62.5-4000pg/mlSensitivity:<37.5pg/mlPrecision: .Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to BAFF. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to BAFF. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BAFF , Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BAFF in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:*354179A: Microtiter Strips, 8x12 wells.*354179B: Standard, 2 vials354179C: Sample/Standard Dilution Buffer, 1x20ml354179D: Antibody-Biotin (Concentrated), 1x120ul354179E: Antibody Dilution Buffer, 1x10ml354179F: Streptavidin-HRP Conjugate (SABC), 1x120ul354179G: SABC Dilution Buffer, 1x10ml354179H: TMB Substrate, 1x10ml354179J: Stop Solution, 1x10ml354179K: Wash Buffer (25X), 1x30mlStorage and Stability:Store unopened *354179A at 4oC; store at -20oC once opened. Store unopened *354179B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Unused kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
- 產(chǎn)品特點(diǎn)
- The BAFF (B-Cell Activating Factor) BioAssay ELISA Kit (Human) is a quantitative sandwich assay for the detection of BAFF in human serum, plasma, tissue homogenates and other biological fluids.Detection Range:62.5-4000pg/mlSensitivity:<37.5pg/mlPrecision: .Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to BAFF. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to BAFF. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BAFF , Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BAFF in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:*354179A: Microtiter Strips, 8x12 wells.*354179B: Standard, 2 vials354179C: Sample/Standard Dilution Buffer, 1x20ml354179D: Antibody-Biotin (Concentrated), 1x120ul354179E: Antibody Dilution Buffer, 1x10ml354179F: Streptavidin-HRP Conjugate (SABC), 1x120ul354179G: SABC Dilution Buffer, 1x10ml354179H: TMB Substrate, 1x10ml354179J: Stop Solution, 1x10ml354179K: Wash Buffer (25X), 1x30mlStorage and Stability:Store unopened *354179A at 4oC; store at -20oC once opened. Store unopened *354179B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Unused kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
- 保存建議
- 建議收到BAFF (B-Cell Activating Factor) BioAssay ELISA Kit (Human)產(chǎn)品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
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