- 中文名稱
Corticosterone (CORT) BioAssay ELISA Kit (Rat)
- 英文名字
- Corticosterone (CORT) BioAssay ELISA Kit (Rat)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號
- 354930
- 產(chǎn)品報價
- ￥1/96Tests
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊、技術(shù)支持團(tuán)隊、市場營銷團(tuán)隊、進(jìn)出口報關(guān)團(tuán)隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- The Corticosterone (CORT) BioAssay ELISA Kit is a quantitative competitive assay for the detection of CORT in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range:2.81-180ng/mlSensitivity:<1.688ng/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with CORT. CORT in the standard or sample competes with a fixed amount of plate-coated CORT for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of CORT in the samples is determined by comparing the absorbance of the samples to the standard curve.Kit Components:*354930A: Microtiter Strips, 1x96 wells (8x12 wells).*354930B: Standard, 2x1 vial354930C: Sample/Standard Dilution Buffer, 1x20ml354930D: Antibody-Biotin (Concentrated), 1x60ul354930E: Antibody Dilution Buffer, 1x10ml354930F: Streptavidin (HRP) (SABC,) 1x120ul354930G: SABC Dilution Buffer, 1x10ml354930H: TMB Substrate, 1x10ml354930J: Stop Solution, 1x10ml354930K: Wash Buffer, 25X, 1x30mlStorage and Stability:Store unopened *354930A at 4oC; store at -20oC once opened. Store unopened *354930 at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards and samples to wells!2. Add 50ul standard or sample to each well.3. Add 50ul Antibody (Biotin) working solution to each well and incubate for 45 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results
- 產(chǎn)品特點(diǎn)
- The Corticosterone (CORT) BioAssay ELISA Kit is a quantitative competitive assay for the detection of CORT in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range:2.81-180ng/mlSensitivity:<1.688ng/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with CORT. CORT in the standard or sample competes with a fixed amount of plate-coated CORT for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of CORT in the samples is determined by comparing the absorbance of the samples to the standard curve.Kit Components:*354930A: Microtiter Strips, 1x96 wells (8x12 wells).*354930B: Standard, 2x1 vial354930C: Sample/Standard Dilution Buffer, 1x20ml354930D: Antibody-Biotin (Concentrated), 1x60ul354930E: Antibody Dilution Buffer, 1x10ml354930F: Streptavidin (HRP) (SABC,) 1x120ul354930G: SABC Dilution Buffer, 1x10ml354930H: TMB Substrate, 1x10ml354930J: Stop Solution, 1x10ml354930K: Wash Buffer, 25X, 1x30mlStorage and Stability:Store unopened *354930A at 4oC; store at -20oC once opened. Store unopened *354930 at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards and samples to wells!2. Add 50ul standard or sample to each well.3. Add 50ul Antibody (Biotin) working solution to each well and incubate for 45 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results
- 保存建議
- 建議收到Corticosterone (CORT) BioAssay ELISA Kit (Rat)產(chǎn)品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。
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