PDGF-BB (Platelet-Derived Growth Factor-BB) BioAssay ELISA Kit (Mouse)-PDGF-BB(Platelet-Derived Growth Factor-BB) BioAssay ELISA Kit(Mouse)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: PDGF-BB (Platelet-Derived Growth Factor-BB) BioAssay ELISA Kit (Mouse)

英文名字: PDGF-BB(Platelet-Derived Growth Factor-BB) BioAssay ELISA Kit(Mouse)

供應商: USBiological

產(chǎn)品貨號: 357966

產(chǎn)品報價: ￥1/96Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: The Mouse Platelet-Derived Growth Factor-BB （PDGF-BB） BioAssay ELISA Kit is a quantitative sandwich assay for the detection of PDGF-BB in mouse serum，plasma，tissue homogenates and other biological fluids.Detection Range：39.063-2500pg/mlSensitivity：<23.438pg/mlAssay Principle：The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDGF-BB. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to PDGF-BB. Streptavidin conjugated to Horseradish Peroxidase （HRP） is added to each microplate well and incubated. After TMB substrate solution is added，only those wells that contain PDGF-BB，Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDGF-BB in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components：*357966A： Microtiter Strips，8x12 wells.*357966B： Standard，2 vials357966C： Sample/Standard Dilution Buffer，1x20ml357966D： Antibody-Biotin （Concentrated），1x120ul357966E： Antibody Dilution Buffer，1x10ml357966F： Streptavidin-HRP Conjugate （SABC），1x120ul357966G： SABC Dilution Buffer，1x10ml357966H： TMB Substrate，1x10ml357966J： Stop Solution，1x10ml357966K： Wash Buffer （25X），1x30mlStorage and Stability：Store unopened *357966A at 4oC; store at -20oC once opened. Store unopened *357966B at 4°C; once reconstituted，store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product，centrifuge the original vials after thawing and prior to removing the cap.Assay Summary：1. Wash plate 2 times before adding standards，samples and control （zero） to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.

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保存建議: 建議收到PDGF-BB （Platelet-Derived Growth Factor-BB） BioAssay ELISA Kit （Mouse）產(chǎn)品后將其置于4°C至-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。