Pregnenolone BioAssay ELISA Kit (Universal)-Pregnenolone BioAssay ELISA Kit(Universal)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Pregnenolone BioAssay ELISA Kit (Universal)

英文名字: Pregnenolone BioAssay ELISA Kit(Universal)

供應商: USBiological

產品貨號: 358304

產品報價: ￥1/96Tests

產品說明書: 點擊查看

購買方式: 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868

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背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業(yè)銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應，專業(yè)的產品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！

產品描述: The Pregnenolone BioAssay ELISA Kit （Universal） is a quantitative competitive assay for the detection of Pregnenolone in serum，plasma，tissue homogenates and other biological fluids.Detection Range：15.6-1,000pg/mlSensitivity：<9.375pg/mlPrecision：Intra-Assay CV： <8%Inter-Assay CV： <10%Assay Principle：The microtiter plate provided in this kit has been pre-coated with Pregnenolone. Pregnenolone in the standard or sample competes with a fixed amount of plate-coated Pregnenolone for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate，after which Streptavidin-HRP （SABC） is added to each well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of Pregnenolone in the samples is determined by comparing the absorbance of the samples to the standard curve.Kit Components：*358304A： Microtiter Strips，8x12 wells.*358304B： Standard，2 vials358304C： Sample/Standard Dilution Buffer，1x20ml358304D： Antibody-Biotin Concentrate，1x160ul358304E： Antibody Dilution Buffer，1x10ml358304F： Streptavidin-HRP Conjugate （SABC），1x120ul358304G： SABC Dilution Buffer，1x10ml358304H： TMB Substrate，1x10ml358304J： Stop Solution，1x10ml358304K： Wash Buffer （25X），1x30mlStorage and Stability：Store unopened *358304A at 4oC; store at -20oC once opened. Store unopened *358304B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product，centrifuge the original vials after thawing and prior to removing the cap.Assay Summary：1. Wash plate 2 times before adding standards，samples and control （zero） to wells!2. Add 50ul standard or sample to each well.3. Immediately add 50ul Antibody-Biotin working solution to each well and incubate for 45 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-20 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.

產品特點: 針對Pregnenolone BioAssay ELISA Kit (Universal)該產品的特點優(yōu)勢，歡迎查閱官網提供的產品說明書。

保存建議: 建議收到Pregnenolone BioAssay ELISA Kit （Universal）產品后將其置于4°C至-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。