The Selenoprotein P (SEPP1) BioAssay ELISA Kit (Rat) is a quantitative sandwich assay for the detection of SEPP1 in rat serum,plasma,tissue homogenates and other biological fluids.Detection Range:0.156-10ng/mlSensitivity:<0.094ng/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Assay Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEPP1. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to SEPP1. Streptavidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,only those wells that contain SEPP1,biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SEPP1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:*358784A: Microtiter Strips,8x12 wells.*358784B: Standard,2 vials358784C: Sample/Standard Dilution Buffer,1x20ml358784D: Antibody-Biotin (Concentrated),1x120ul358784E: Antibody Dilution Buffer,1x10ml*358784F: Streptavidin-HRP Conjugate (SABC),1x120ul358784G: SABC Dilution Buffer,1x10ml*358784H: TMB Substrate,1x10ml358784J: Stop Solution,1x10ml358784K: Wash Buffer (25X),1x30mlStorage and Stability:Store unopened *358784A at 4oC; store at -20oC once opened. Store unopened *358784B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store *358784F and *358784H protected from light at 4°C. Store all other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product,centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards,samples and control (zero) to wells.2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C 3. Aspirate the liquid and wash plate 3 times.3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate the liquid and wash plate 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate the liquid and wash plate 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
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保存建議
建議收到Selenoprotein P (SEPP1) BioAssay ELISA Kit (Rat)產(chǎn)品后將其置于4°C至-20°C保存。