IL-12 p70 BioAssay ELISA Kit (Human) (Interleukin-12 subunit alpha，IL-12A，Cytotoxic lymphocyte maturation factor 35kD subunit，CLMF p35，IL-12 subunit p35，NK cell stimulatory factor chain 1，IL12A，NKSF1，-IL-12 p70 BioAssay ELISA Kit(Human)(Interleukin-12 subunit alpha，IL-12A，Cytotoxic lymphocyte maturation factor 35kD subunit，CLMF p35，IL-12 subunit p35，NK cell stimulatory factor chain 1，IL12A，NKSF1，Interleukin-12 subunit beta，IL-12B，Cytotoxic lymphocyte maturation factor 40kD)代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: IL-12 p70 BioAssay ELISA Kit (Human) (Interleukin-12 subunit alpha，IL-12A，Cytotoxic lymphocyte maturation factor 35kD subunit，CLMF p35，IL-12 subunit p35，NK cell stimulatory factor chain 1，IL12A，NKSF1，

英文名字: IL-12 p70 BioAssay ELISA Kit(Human)(Interleukin-12 subunit alpha，IL-12A，Cytotoxic lymphocyte maturation factor 35kD subunit，CLMF p35，IL-12 subunit p35，NK cell stimulatory factor chain 1，IL12A，NKSF1，Interleukin-12 subunit beta，IL-12B，Cytotoxic lymphocyte maturation factor 40kD)

供應(yīng)商: USBiological

產(chǎn)品貨號: 360475

產(chǎn)品報(bào)價(jià): ￥1/96Tests

產(chǎn)品說明書: 點(diǎn)擊查看

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產(chǎn)品描述: Interleukin 12 （IL-12），also known as natural killer cell stimulatory factor （NKSF） or cytotoxic lymphocyte maturation factor （CLMF），is a heterodimeric pleiotropic cytokine made up of a 40kD （p40） subunit and a 35kD （p35） subunit. The IL-12 p40 subunit is shared by IL-23，another heterodimeric cytokine that has biological activities similar to，as well as distinct from，IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer （NK） cells. While mouse IL-12 is active on both human and mouse cells，human IL-12 is not active on mouse cells.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human IL-12 p70 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-12 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：30pg/mlRange：62.5-4000pg/mlSpecificity：Recognizes human IL-12.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h IL-12 monoclonal coating antibody is adsorbed onto microwells. IL-12 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-12 antibody is added and binds to IL-12 captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-12 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-12 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-12 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12 standard dilutions and IL-12 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x12Standard： 2xVialBiotinylated antibody： 2xVialStreptavidin-HRP： 2xVialAssay Buffer （25ml/Vial）： 2xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x12mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 3xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.

產(chǎn)品特點(diǎn): 針對IL-12 p70 BioAssay ELISA Kit (Human) (Interleukin-12 subunit alpha，IL-12A，Cytotoxic lymphocyte maturation factor 35kD subunit，CLMF p35，IL-12 subunit p35，NK cell stimulatory factor chain 1，IL12A，NKSF1，Interleukin-12 subunit beta，IL-12B，Cyto該產(chǎn)品的特點(diǎn)優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。

保存建議: 建議收到IL-12 p70 BioAssay ELISA Kit （Human）（Interleukin-12 subunit alpha，IL-12A，Cytotoxic lymphocyte maturation factor 35kD subunit，CLMF p35，IL-12 subunit p35，NK cell stimulatory factor chain 1，IL12A，NKSF1，Interleukin-12 subunit beta，IL-12B，Cytotoxic lymphocyte maturation factor 40kD）產(chǎn)品后將其置于4°C至-20°C保存。

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