IL-4 BioAssay ELISA Kit (Human) (Interleukin-4，IL-4，B-cell stimulatory factor 1，BSF-1，Binetrakin，Lymphocyte stimulatory factor 1，Pitrakinra，IL4)-IL-4 BioAssay ELISA Kit(Human)(Interleukin-4，IL-4，B-cell stimulatory factor 1，BSF-1，Binetrakin，Lymphocyte stimulatory factor 1，Pitrakinra，IL4)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: IL-4 BioAssay ELISA Kit (Human) (Interleukin-4，IL-4，B-cell stimulatory factor 1，BSF-1，Binetrakin，Lymphocyte stimulatory factor 1，Pitrakinra，IL4)

英文名字: IL-4 BioAssay ELISA Kit(Human)(Interleukin-4，IL-4，B-cell stimulatory factor 1，BSF-1，Binetrakin，Lymphocyte stimulatory factor 1，Pitrakinra，IL4)

供應商: USBiological

產品貨號: 360515

產品報價: ￥1/48Tests

產品說明書: 點擊查看

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產品描述: Interleukin 4 （IL-4） is a pleiotropic cytokine produced by activated T cells，mast cells，and basophils. It was initially identified as a B cell differentiation factor （BCDF），as well as a B cell stimulatory factor （BSF1）. IL-4 has since been shown to have multiple biological effects on hematopoietic and non-hematopoietic cells，including B and T cells，monocytes，macrophages，mast cells，myeloid and erythroid progenitors，fibroblasts，and endothelial cells. Rat，mouse and human IL-4 are species-specific in their activities.Sample Type：Cell culture supernatants，serum，plasma，and tissueIntended Use：The Human IL-4 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Human IL-4 concentrations in cell culture supernatants，serum，plasma，and tissue.Sensitivity：2pg/mlRange：3.9-250pg/mlSpecificity：Recognizes human IL-4.Sample Volume：100ul/wellTest Principle：This assay employs the Sandwich immunoassay technique. An anti-h IL-4 monoclonal coating antibody is adsorbed onto microwells. IL-4 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-h IL-4 antibody is added and binds to IL-4 captured by the first antibody. Following incubation unbound Biotinylated anti-h IL-4 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-h IL-4 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-4 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-4 standard dilutions and IL-4 sample concentration determined.Kit Components：Aluminium pouches with a Microwell Plate： 8x6Standard： 1xVialBiotinylated antibody： 1xVialStreptavidin-HRP： 1xVialAssay Buffer （25ml/Vial）： 1xVialWash Buffer Concentrate 20x （30ml/Vial）： 1xVialTMB Substrate Solution： 1x6mlStop Solution （12ml/Vial）： 1xVialPlate Covers- Adhesive strips： 2xstripsInstruction： 1xStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.

產品特點: 針對IL-4 BioAssay ELISA Kit (Human) (Interleukin-4，IL-4，B-cell stimulatory factor 1，BSF-1，Binetrakin，Lymphocyte stimulatory factor 1，Pitrakinra，IL4)該產品的特點優(yōu)勢，歡迎查閱官網提供的產品說明書。

保存建議: 建議收到IL-4 BioAssay ELISA Kit （Human）（Interleukin-4，IL-4，B-cell stimulatory factor 1，BSF-1，Binetrakin，Lymphocyte stimulatory factor 1，Pitrakinra，IL4）產品后將其置于4°C至-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。