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細(xì)胞分析試劑盒
中文名稱

IL-6 BioAssay ELISA Kit (Mouse) (Interleukin-6,IL-6,B-cell stimulatory factor 2,CTL differentiation factor,CDF,Hybridoma growth factor,Interferon beta-2,IFN-beta-2,IL6,IFNB2)

英文名字
IL-6 BioAssay ELISA Kit(Mouse)(Interleukin-6,IL-6,B-cell stimulatory factor 2,CTL differentiation factor,CDF,Hybridoma growth factor,Interferon beta-2,IFN-beta-2,IL6,IFNB2)
供應(yīng)商
USBiological
產(chǎn)品貨號(hào)
360527
產(chǎn)品報(bào)價(jià)
¥1/48Tests
產(chǎn)品說明書
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產(chǎn)品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理,是行業(yè)中少有的致力于服務(wù)客戶,幫助客戶,且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng),專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù),在意您的成功!
產(chǎn)品描述
Interleukin 6 (IL-6) is a multifunctional cytokine that plays important roles in host defense,acute phase reactions,immune responses,nerve cell functions and hematopoiesis. It is expressed by a variety of normal and transformed lymphoid and nonlymphoid cells. The production of IL-6 is upregulated by numerous signals such as mitogenic or antigenic stimulation,lipopolysaccharides,calcium ionophores,cytokines and viruses. IL-4,IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions,including bacterial and viral infections,trauma,autoimmune diseases,inflammations and malignancies. The mouse IL-6 cDNA encodes a 211aa residue precursor polypeptide with a hydrophobic signal sequence that is cleaved to generate the 187aa residue mature protein. Mouse to rat and human,there is approximately 87% and 39%aa identity,respectively. Although human and mouse IL-6 are equally active on mouse cells,mouse IL-6 is not active on human cells.Sample Type:Cell culture supernatants,serum,plasma,and tissueIntended Use:The Mouse IL-6 BioAssay ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of Mouse IL-6 concentrations in cell culture supernatants,serum,plasma,and tissue.Sensitivity:7pg/mlRange:15.625-1000pg/mlSpecificity:Recognizes mouse IL-6.Sample Volume:100ul/wellTest Principle:This assay employs the Sandwich immunoassay technique. An anti-m IL-6 monoclonal coating antibody is adsorbed onto microwells. IL-6 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-m IL-6 antibody is added and binds to IL-6 captured by the first antibody. Following incubation unbound Biotinylated anti-m IL-6 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-m IL-6 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-6 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-6 standard dilutions and IL-6 sample concentration determined.Kit Components:Aluminium pouches with a Microwell Plate: 8x6Standard: 1xVialBiotinylated antibody: 1xVialStreptavidin-HRP: 1xVialAssay Buffer (25ml/Vial): 1xVialWash Buffer Concentrate 20x (30ml/Vial): 1xVialTMB Substrate Solution: 1x6mlStop Solution (12ml/Vial): 1xVialPlate Covers- Adhesive strips: 2xstripsInstruction: 1xStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product,centrifuge the original vial after thawing and prior to removing the cap.
產(chǎn)品特點(diǎn)
針對(duì)IL-6 BioAssay ELISA Kit (Mouse) (Interleukin-6,IL-6,B-cell stimulatory factor 2,CTL differentiation factor,CDF,Hybridoma growth factor,Interferon beta-2,IFN-beta-2,IL6,IFNB2)該產(chǎn)品的特點(diǎn)優(yōu)勢(shì),歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
保存建議
建議收到IL-6 BioAssay ELISA Kit (Mouse) (Interleukin-6,IL-6,B-cell stimulatory factor 2,CTL differentiation factor,CDF,Hybridoma growth factor,Interferon beta-2,IFN-beta-2,IL6,IFNB2)產(chǎn)品后將其置于4°C至-20°C保存。
其他
Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶提供了諸多超值選擇。
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