Sulforhodamine B Cell Cytotoxicity，Colorimetric BioAssay Kit (SRB)-Sulforhodamine B Cell Cytotoxicity，Colorimetric BioAssay Kit(SRB)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Sulforhodamine B Cell Cytotoxicity，Colorimetric BioAssay Kit (SRB)

英文名字: Sulforhodamine B Cell Cytotoxicity，Colorimetric BioAssay Kit(SRB)

供應商: USBiological

產(chǎn)品貨號: 369292

產(chǎn)品報價: ￥1/1000Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: Intended Use：This SRB cell cytotoxicity BioAssay kit is simple，accurate，reproducible and sensitive. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening that can detect between 5000-50,000 cells/well. This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. Relies on the ability of SRB to bind cellular protein components and measure the total biomass.Sample Type：Cell culture： Adherent cells.Range： 5000-50,000 cells/wellSpecificity：MammalianTest Principle：This Sulforhodamine B （SRB） cell cytotoxicity BioAssay is one of the most widely methods used to detect cell viability or drug cytotoxicity. This assay relies on the ability of SRB to bind cellular protein components and measure the total biomass. SRB is a bright-pink aminoxanthene dye that can form an electrostatic complex with basic amino acid residues of proteins in slightly acidic conditions but it can dissociate under basic conditions. It has been widely used for drug toxicity screening against different types of cancerous and non-cancerous cell lines. In addition，this assay is independent of cell metabolic activity and therefore should show less interference by the testing compounds. Since the binding of SRB is stoichiometric，the incorporated dye released from stained cells after washing is directly proportional to the cell biomass and can be measured at 565nm.Kit Components：Fixation Solution，1x55ml20X Washing Solution，1x50ml10X Solubilization Solution，1x22mlSRB Dye Solution，1x50ml20mM Doxorubicin，1x100ulStorage and Stability：Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product，centrifuge the original vial after thawing and prior to removing the cap.

產(chǎn)品特點: 針對Sulforhodamine B Cell Cytotoxicity，Colorimetric BioAssay Kit (SRB)該產(chǎn)品的特點優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。

保存建議: 建議收到Sulforhodamine B Cell Cytotoxicity，Colorimetric BioAssay Kit （SRB）產(chǎn)品后將其置于-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。