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細(xì)胞分析試劑盒
中文名稱(chēng)

APP BioAssay ELISA Kit (Monkey)

英文名字
APP BioAssay ELISA Kit (Monkey)
供應(yīng)商
USBiological
產(chǎn)品貨號(hào)
396565
產(chǎn)品報(bào)價(jià)
¥1/96Tests
產(chǎn)品說(shuō)明書(shū)
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產(chǎn)品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類(lèi)最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理,是行業(yè)中少有的致力于服務(wù)客戶(hù),幫助客戶(hù),且擁有獨(dú)立的專(zhuān)業(yè)銷(xiāo)售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷(xiāo)團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢(xún)響應(yīng),專(zhuān)業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù),在意您的成功!
產(chǎn)品描述
Amyloid precursor protein(APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid(Abeta), a 39- to 42aa peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha-, beta-, and gamma-secretases. Alpha-secretase generates soluble amyloid protein, while beta- and gamma-secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive.Sample Type:Serum, cell culture supernates, plasma (heparin or EDTA).Intended Use:Sandwich High Sensitivity BioAssay ELISA kit for Quantitative Detection of Monkey APP.Sensitivity:<100pg/mlRange:312pg/ml-20,000pg/mlSpecificity:Recognizes natural and recombinant Monkey APP. There is no detectable cross-reactivity with other relevant proteins.Test Principle:This Monkey APP BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Monkey APP has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: L18-L688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey APP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey APP amount of sample captured in plate.Kit Components:96-well plate precoated with anti-Monkey APP antibody, 1x plateLyophilized recombinant Monkey APP standard, 2x10ng/tubeBiotinylated anti-Monkey APP antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x bufferStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產(chǎn)品特點(diǎn)
針對(duì)APP BioAssay ELISA Kit (Monkey)該產(chǎn)品的特點(diǎn)優(yōu)勢(shì),歡迎查閱官網(wǎng)提供的產(chǎn)品說(shuō)明書(shū)。
保存建議
建議收到APP BioAssay ELISA Kit (Monkey)產(chǎn)品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類(lèi)最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶(hù)提供了諸多超值選擇。
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