BMP-5 BioAssay ELISA Kit (Bone Morphogenetic Protein 5) (Equine)-BMP-5 BioAssay ELISA Kit (Bone Morphogenetic Protein 5) (Equine)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: BMP-5 BioAssay ELISA Kit (Bone Morphogenetic Protein 5) (Equine)

英文名字: BMP-5 BioAssay ELISA Kit (Bone Morphogenetic Protein 5) (Equine)

供應(yīng)商: USBiological

產(chǎn)品貨號: 396581

產(chǎn)品報價: ￥1/96Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's, BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on equine chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18kD.Sample Type:Cell culture supernates, serum, bone tissue, tissue.Intended Use:Sandwich High Sensitivity ELISA kit for Quantitative Detection of Equine BMP-5. Sensitivity:<10pg/mlRange:156pg/ml-10000g/mlSpecificity:Recognizes natural and recombinant equine BMP-5. There is no detectable cross-reactivity with other relevant proteins.Test Principle:This Equine BMP-5 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Equine BMP-5 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A317-H454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Equine BMP-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 216 Equine BMP-5 amount of sample captured in plate.Kit Components:96-well plate precoated with anti-Equine BMP-5 antibody, 1x plateLyophilized recombinant Equine BMP-5 standard, 2x10ng/tubeBiotinylated anti-Equine BMP-5 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x bufferStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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保存建議: 建議收到BMP-5 BioAssay ELISA Kit (Bone Morphogenetic Protein 5) (Equine)產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。