MMP-14 BioAssay ELISA Kit (Human)-MMP-14 BioAssay ELISA Kit (Human)代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: MMP-14 BioAssay ELISA Kit (Human)

英文名字: MMP-14 BioAssay ELISA Kit (Human)

供應(yīng)商: USBiological

產(chǎn)品貨號(hào): 396722

產(chǎn)品報(bào)價(jià): ￥1/96Tests

產(chǎn)品說(shuō)明書(shū): 點(diǎn)擊查看

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產(chǎn)品描述: Matrix metalloproteinase-14 is an enzyme that in humans is encoded by the MMP14 gene. It is mapped to chromosome 14q11-q12. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive pro-proteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.Sample Type:Cell culture supernates, serum, plasma (heparin or EDTA).Intended Use:Sandwich High Sensitivity BioAssay ELISA kit for Quantitative Detection of Human MMP-14.Sensitivity:<15pg/mlRange:156pg/ml-10000pg/mlSpecificity:Recognizes natural and recombinant Human MMP-14. There is no detectable cross-reactivity with other relevant proteins.Test Principle:This Human MMP-14 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for MMP-14 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A21-S538) and test samples are added to the wells, a biotinylated detection specific for MMP-14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MMP-14 amount of sample captured in plate.Kit Components:96-well plate precoated with anti-Human MMP14 antibody, 1x plateLyophilized recombinant Human MMP14 standard, 2x10ng/tubeBiotinylated anti-Human MMP14 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x bufferStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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保存建議: 建議收到MMP-14 BioAssay ELISA Kit (Human)產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。