SDF-1 (Stromal Cell Derived Factor 1) BioAssay ELISA Kit (Monkey)-SDF-1 (Stromal Cell Derived Factor 1) BioAssay ELISA Kit (Monkey)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: SDF-1 (Stromal Cell Derived Factor 1) BioAssay ELISA Kit (Monkey)

英文名字: SDF-1 (Stromal Cell Derived Factor 1) BioAssay ELISA Kit (Monkey)

供應商: USBiological

產(chǎn)品貨號: 396758

產(chǎn)品報價: ￥1/96Tests

產(chǎn)品說明書: 點擊查看

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背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業(yè)銷售團隊、技術(shù)支持團隊、市場營銷團隊、進出口報關(guān)團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務。我們致力于為您提供有價值的產(chǎn)品和服務，在意您的成功！

產(chǎn)品描述: SDF-1(stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine(C-X-C motif) ligand 12(CXCL12). This gene is located on chromosome 10q11.1. SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening aa. CXCL12 is strongly chemotactic for lymphocytes. CXCL12 was shown to be expressed in many tissues in mice(including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow). CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes. CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide. The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8kD.Sample Type:Cell culture supernates, serum, plasma (heparin).Intended Use:Sandwich High Sensitivity BioAssay ELISA kit for Quantitative Detection of Monkey SDF-1.Sensitivity:<10pg/mlRange:62.5pg/ml-4000pg/mlSpecificity:Recognizes natural and recombinant Monkey SDF-1. There is no detectable cross-reactivity with other relevant proteins.Test Principle:This Monkey SDF-1 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Monkey SDF-1 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: K22-M93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey primate SDF-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey SDF-1 amount of sample captured in plate.Kit Components:96-well plate precoated with anti-Monkey SDF-1 antibody, 1x plateLyophilized recombinant Monkey SDF-1 standard, 2x10ng/tubeBiotinylated anti-Monkey SDF-1 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x bufferStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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保存建議: 建議收到SDF-1 (Stromal Cell Derived Factor 1) BioAssay ELISA Kit (Monkey)產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。