Survivin BioAssay ELISA Kit (Monkey)-Survivin BioAssay ELISA Kit (Monkey)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Survivin BioAssay ELISA Kit (Monkey)

英文名字: Survivin BioAssay ELISA Kit (Monkey)

供應商: USBiological

產(chǎn)品貨號: 396768

產(chǎn)品報價: ￥1/96Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: Survivin(also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common Monkey cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50% of high-grade non- Hodgkin's lymphomas. Survivin encodes a deduced 142aa protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant Monkey Survivin with the molecular mass of 34kD.Sample Type:Serum, cell culture supernates, plasma (heparin or EDTA).Intended Use:Sandwich High Sensitivity BioAssay ELISA kit for Quantitative Detection of Monkey Survivin.Sensitivity:<2pg/mlRange:62.5pg/ml-4000pg/mlSpecificity:Recognizes natural and recombinant Monkey Survivin. There is no detectable cross-reactivity with other relevant proteins.Test Principle:This Monkey Survivin BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey Survivin has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: M1-D142) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey Survivin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey Survivin amount of sample captured in plate.Kit Components:96-well plate precoated with anti-Monkey Survivin antibody, 1x plateLyophilized recombinant Monkey Survivin standard, 2x10ng/tubeBiotinylated anti-Monkey Survivin antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x bufferStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產(chǎn)品特點: 針對Survivin BioAssay ELISA Kit (Monkey)該產(chǎn)品的特點優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。

保存建議: 建議收到Survivin BioAssay ELISA Kit (Monkey)產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。