TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) BioAssay ELISA Kit (Porcine)-TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) BioAssay ELISA Kit (Porcine)代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) BioAssay ELISA Kit (Porcine)

英文名字: TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) BioAssay ELISA Kit (Porcine)

供應(yīng)商: USBiological

產(chǎn)品貨號(hào): 396794

產(chǎn)品報(bào)價(jià): ￥1/96Tests

產(chǎn)品說(shuō)明書(shū): 點(diǎn)擊查看

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背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！

產(chǎn)品描述: The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).Sample Type:Serum, cell culture supernates, saliva, plasma (heparin or EDTA).Intended Use:Sandwich High Sensitivity BioAssay ELISA kit for Quantitative Detection of Porcine TIMP-3.Sensitivity:<2pg/mlRange:156pg/ml-10,000pg/mlSpecificity:Recognizes natural and recombinant Porcine TIMP-3. There is no detectable cross-reactivity with other relevant proteins.Test Principle:This Porcine TIMP-3 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Porcine TIMP-3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: C24-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Porcine TIMP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine TIMP-3 amount of sample captured in plate.Kit Components:96-well plate precoated with anti-Porcine TIMP3 antibody, 1x plateLyophilized recombinant Porcine TIMP3 standard, 2x10ng/tubeBiotinylated anti-Porcine TIMP3 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x bufferStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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保存建議: 建議收到TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) BioAssay ELISA Kit (Porcine)產(chǎn)品后將其置于4°C/-20°C保存。

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