Glutathione S-transferase (GST) is a widely used protein fusion partner,since it provides an easily detectable Tag and also a simple purification process with little effect on the biological function of the protein of interest.
應(yīng)用類型
Western blotting (0.5-4 ug/ml) and Immunohistochemistry (10-20 ug/ml, frozen & paraffin). Recombinant GST-GSK-3 fusion protein (Cat.#: 7003-100) can be used as a positive control. However, the optimal conditions should be determined individually.