Glial cell line-derived neurotrophic factor (GDNF) has been identified as a potent neurotrophic factor that enhances survival of midbrain dopaminergic neurons. GDNF is a glycosylated,disulfide-bonded homodimer. It promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. On the basis of these findings,it has been suggested that GDNF may play a role in the treatment of Parkinson’s disease,which is marked by progressive degeneration of midbrain dopaminergic neurons.
應(yīng)用類型
Western blotting (0.5-4 ug/ml). Based on researcher’s feedback, the antibody also works in immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes GDNF of human, mouse, and rat origins. Jurkat cell lysate can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 195 of mouse GDNF
來源宿主
兔
反應(yīng)性
經(jīng)檢測驗證,可與人、小鼠、大鼠種屬來源的GDNF蛋白發(fā)生免疫反應(yīng).
保存建議
提供的GDNF抗體產(chǎn)品為100 ug 無色溶液,其濃度為0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS緩沖液中.請將該GDNF抗體產(chǎn)品置于-20 ℃冷凍保存,建議分裝使用以避免反復(fù)凍融.