SDF-1 (stromal cell-derived factor-),also known as pre-B-cell growth-stimulating factor (PBSF),is a 70-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis. SDF-1 has been shown to be chemotactic for lymphocytes. In addition,SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin),a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry.
應(yīng)用類型
Western blot analysis (0.5-1 ug/ml). Based on researcher’s feedback, it can also be used in immunofluorescence and neutralization (2-10 ug/ml). However, the optimal conditions should be determined individually.