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中文名稱(chēng)

骨橋蛋白(N末端)(克隆號(hào)2E11)

英文名字
Anti-Osteopontin (NT) (Clone 2E11) (OPN, BNSP, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin.)
供應(yīng)商
Biovision
產(chǎn)品貨號(hào)
5424-100
產(chǎn)品報(bào)價(jià)
¥已停產(chǎn)
產(chǎn)品說(shuō)明書(shū)
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產(chǎn)品新聞
背景資料
Human Osteopontin (OPN) is a negatively charged hydrophilic protein of 314 amino acids and is subject to significant post translational modifications (PTM) including phosphorylation and glycosylation. Due to its acidic nature and PTMs,OPN runs anomalously by SDS-PAGE. Although its mass is 35kD,apparent molecular weights may range up to 75kD. In addition,OPN is subject to proteolytic modification into smaller molecular weight fragments. The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk,blood and urine,depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids,and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children,serum levels of osteopontin are correlated to the severity of disease,probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis,increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker,since it is associated with tumor formation,progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor,which induces osteogenesis. Without appropriate levels of osteopontin,bone growth continues unregulated,and leads to specific bone cancers. In short,osteopontin is a strong marker for bone growth,inflammation and certain cancers. The newly exposed SVVYG epitope on the N-terminal fragment has also been shown to participate in cell adhesion.
應(yīng)用類(lèi)型
ELISA, Western Blot.
免疫原
Recombinant full-length human Osteopontin
來(lái)源宿主
小鼠
反應(yīng)性
經(jīng)檢測(cè)驗(yàn)證,可與人(奶源中的天然OPN蛋白)種屬來(lái)源的Osteopontin (N末端) (克隆號(hào)2E11)蛋白發(fā)生免疫反應(yīng).
保存建議
提供的Osteopontin (N末端) (克隆號(hào)2E11)產(chǎn)品為無(wú)色溶液形式,含有100 ug抗體.該產(chǎn)品濃度為1 mg/ml,溶解于0.15 M PBS緩沖液中.請(qǐng)?jiān)谑盏皆揙steopontin (N末端) (克隆號(hào)2E11)產(chǎn)品后,將其置于-20 °C冷凍保存,建議分裝使用以避免反復(fù)凍融.
其他
BioVision的公司總部在美麗的舊金山灣地區(qū),它是全球生命科學(xué)公司的Leader,BioVision在開(kāi)發(fā)和研究細(xì)胞凋亡檢測(cè)分析、代謝檢測(cè)方面具有卓越的影響力.包括檢測(cè)各種檢測(cè)分析試劑盒、相關(guān)特色抗體、重組蛋白及細(xì)胞因子和酶等多種產(chǎn)品,以及其他創(chuàng)新的研究工具.其產(chǎn)品覆蓋細(xì)胞凋亡研究、能量代謝、細(xì)胞/組織蛋白提取、氧化應(yīng)激與損傷、代謝與肥胖癥、細(xì)胞增殖與毒性、信號(hào)轉(zhuǎn)導(dǎo)、干細(xì)胞研究等領(lǐng)域.作為多家知名品牌的中國(guó)總代理以及中國(guó)區(qū)域代理,艾美捷與BioVision一起為研究者們提供優(yōu)質(zhì)、新的細(xì)胞生物學(xué)研究工具而不斷擴(kuò)大產(chǎn)品及服務(wù)質(zhì)量.
注意
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