The mammalian Matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. After cleavage of a single peptide domain of about 20 amino acids,the MMPs are secreted in latent forms. Upon activation,the N-terminal propeptide domain is cleaved to generate the active forms of MMP. MMP-9 (92 kDa type IV collagenase,Gelatinase-B) contains the basic structure of propeptide,catalytic,and hemopexin domains. It is an important proteinase in tissue remodeling.
應(yīng)用類型
Western blot analysis (0.5-4 ug/ml), immunoprecipitation (4-8 ug/ml), and Immunohistochemistry (20-40 ug/ml). However, the optimal conditions should be determined individually.
免疫原
E. coli-expressed active rat 92-kDa type IV collagenase
來源宿主
兔
反應(yīng)性
經(jīng)檢測驗證,可與大鼠種屬來源的MMP-9蛋白發(fā)生免疫反應(yīng).
保存建議
提供的MMP-9抗體產(chǎn)品為100 ug 無色溶液,其濃度為0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)緩沖液中.請將該MMP-9抗體產(chǎn)品置于-20 ℃冷凍保存,建議分裝使用以避免反復凍融.