MeCP2（aa 11-25； aa 181-195）抗體-MeCP2 （aa 11-25； aa 181-195） Antibody （Methyl-CpG-Binding Protein 2）代理/參數(shù)/廠家-二抗-艾美捷科技有限公司

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背景資料: DNA methylation，or the addition of methyl groups to cytosine bases in the dinucleotide CpG，is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. A demethylase enzyme has been identified which exhibits demethylase activity associated to a methyl-CpG-binding domain （MBD）. The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 （PCM1） repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 （also known as MED1） co-localize with foci of heavily methylated satellite DNA and mediate the biological functions of the methylation signal. Surprisingly，MBD3 does not bind methylated DNA either in vivo or in vitro. MeCP2，MBD1，MBD2，MBD3，and MBD4 are expressed in somatic tissues，but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells，which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

應(yīng)用類型: Western blot analysis （2-4 ug/ml）. However， the optimal conditions should be determined individually.

免疫原: Synthetic peptides corresponding to amino acids 11-25 and 181-195 of human MeCP2

反應(yīng)性: 經(jīng)檢測驗證，可與人種屬來源的MeCP2 （aa 11-25； aa 181-195）蛋白發(fā)生免疫反應(yīng).

保存建議: 提供的MeCP2 （aa 11-25； aa 181-195）抗體產(chǎn)品為無色溶液形式，含有50 ug抗體.該產(chǎn)品濃度為與批次相關(guān)，溶解于包含有0.2% gelatin和0.05%疊氮化鈉的PBS緩沖液中.請在收到該MeCP2 （aa 11-25； aa 181-195）抗體產(chǎn)品后，將其置于-20 °C冷凍保存，建議分裝使用以避免反復(fù)凍融.

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