UHRF1,also named as ICBP90,NP95 and RNF106,is a putative E3?ubiquitin protein?ligase. It may?participate in methylation dependent transcriptional?regulation. UHRF1 binds to inverted 5’-CCAAT-3’?box 2 in?the TOP2A?promoter,and activates TOP2A?expression. It is important for G1/S?transition and may be involved in?DNA?repair and chromosomal stability. UHRF1’s ability to repress its direct target gene expression is dependent on PHD (UHRF1) binding to unmodified H3R2.
應(yīng)用類型
Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200, IF: 1:20 – 1:50, IP: 1:20 – 1:50, CHIP: 1:20 – 1:50. However, the optimal conditions should be determined individually.