Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFR,which is a cell surface receptor for GDNF. The cDNA encoding GDNFR from human,rat,chicken and mouse have been cloned recently. GDNFR was also termed Ret ligand 1 (RETL1) or TGF-B-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFR-1 recently. GFR-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus,GDNF,GFR and the Ret PTK from a complex to transduce GDNF signal and to mediate GDNF function.
應(yīng)用類型
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic Peptide to amino acids 369-382 of human GFR-1