Fructose 1,6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate. Fructose 1,6-bisphosphate aldolase exists as three forms,the muscle-specific Aldolase A,the liver-specific aldolase B,and the brain-specific aldolase C. Aldolase A,B,and C arose from a common ancestral gene,from which aldolase B first diverged. Aldolase A is one of the most highly conserved enzymes known,with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagon and has been implicated in hereditary fructose intolerance disease. Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells.
應(yīng)用類型
Western blot: ~1:1000, IHC: ~1:10-1:50.
免疫原
This ALDOC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-333 amino acids from the C-terminal region of human ALDOC.
來源宿主
兔
反應(yīng)性
經(jīng)檢測(cè)驗(yàn)證,可與人(預(yù)測(cè)可與小鼠、大鼠和猴)種屬來源的Aldolase C (ALDOC)抗體(C末端)蛋白發(fā)生免疫反應(yīng).
保存建議
提供的Aldolase C (ALDOC)抗體(C末端)產(chǎn)品為無色溶液形式,含有100 ul抗體.該產(chǎn)品濃度為與批次相關(guān),溶解于包含有0.09% (W/V) 疊氮化鈉的PBS緩沖液中.請(qǐng)?jiān)谑盏皆揂ldolase C (ALDOC)抗體(C末端)產(chǎn)品后,將其置于-20 °C冷凍保存,建議分裝使用以避免反復(fù)凍融.