- 中文名稱
Goat TrueBlot? Set (with IP Agarose beads) - 88-1488-31
- 英文名字
- Goat TrueBlot? Set (with IP Agarose beads) - 88-1488-31
- 供應(yīng)商
- Rockland
- 產(chǎn)品貨號(hào)
- 88-1488-31
- 產(chǎn)品報(bào)價(jià)
- ￥詢價(jià)/1Set
- 產(chǎn)品說(shuō)明書(shū)
- 點(diǎn)擊查看
- 購(gòu)買方式
- 90%產(chǎn)品中國(guó)有現(xiàn)貨庫(kù)存。銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- Goat IgG TrueBlot? is a unique horseradish peroxidase conjugated Anti-Goat IgG monoclonal secondary antibody. Goat IgG TrueBlot? enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Goat IgG TrueBlot?, simply substitute the conventional HRP Anti-Goat IgG blotting reagent with Goat IgG TrueBlot? and follow the prescribed protocol for sample preparation and immunoblotting. Goat IgG TrueBlot? is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of goat IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Goat IgG TrueBlot? with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Goat IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.
- 產(chǎn)品描述
- Goat IgG TrueBlot? is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. MB-70 or BSA is not an effective blocker. Goat TrueBlot Set Components: 1. Goat IgG TrueBlot? HRP-conjugated monoclonal secondary antibody reacting with goat IgGs for optimal signal detection in immunoprecipitation/immunoblotting experiments. 2. Anti-Goat Ig IP Beads: 2.5 mL. Binds 1 mg Ig/mL beads. 3. Western blot incubation tray. Special Notes: Upon initial use of the IP beads, we recommend that the vial be inverted several times to get the beads into suspension. We recommend using a large bore pipet to pipet up the liquid for use. For storage of the opened vial of beads, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user.
- 產(chǎn)品特點(diǎn)
- 該Goat TrueBlot? Set (with IP Agarose beads) - 88-1488-31的詳細(xì)信息查看Rockland提供的產(chǎn)品說(shuō)明書(shū)。
- 保存建議
- Store TrueBlot? Anti-Goat Ig IP beads (00-8844-25) at 2-8 °C and Goat TrueBlot? (18-8814-31) at -20 °C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.
- 其他
- Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國(guó)費(fèi)城郊區(qū)的農(nóng)場(chǎng)保有近20種實(shí)驗(yàn)動(dòng)物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號(hào)蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識(shí)別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。
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- 注意
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該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說(shuō)明書(shū)為準(zhǔn)。下單前，請(qǐng)瀏覽說(shuō)明書(shū)確認(rèn)。
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