- 中文名稱
TrueBlot? Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG?) Epitope Tag - 88-8887-383
- 英文名字
- TrueBlot? Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG?) Epitope Tag - 88-8887-383
- 供應(yīng)商
- Rockland
- 產(chǎn)品貨號
- 88-8887-383
- 產(chǎn)品報(bào)價(jià)
- ￥詢價(jià)/1Kit
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 90%產(chǎn)品中國有現(xiàn)貨庫存。銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- The FLAG? epitope is a small but highly immunogenic peptide DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) which allows fusion proteins to retain their original conformation and function. The hydrophilic character of this sequence increases the likelihood that it will be located on the surface of FLAG?-tagged recombinant proteins for reaction with anti-DYKDDDDK (FLAG?) antibodies. The Anti-DYKDDDDK (FLAG?) antibody included in this Western blot kit detects FLAG?-tagged recombinant proteins fused with FLAG? tag either at the amino-terminus or carboxy-terminus of the protein. The TrueBlot? Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG?) Epitope Tag contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples containing the DYKDDDDK (FLAG?) Epitope Tag using TrueBlot monoclonal secondary antibody in conjunction with Rockland's Antibody for the detection of FLAG? conjugated proteins (MOUSE) Monoclonal Antibody (see application notes). Mouse IgG TrueBlot? ULTRA is the unique horseradish peroxidase conjugated Anti-Mouse IgG imonoclonal secondary antibody which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your conventional HRP Anti-Mouse IgG immunoblotting secondary antibody. It is easy to generate publication-quality IP/WB data with Mouse IgG TrueBlot? ULTRA. Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
- 產(chǎn)品描述
- Mouse IgG TrueBlot? ULTRA is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). Mouse IgG TrueBlot? ULTRA is a HRP-conjugated monoclonal secondary antibody reacting with mouse IgGs for optimal signal detection in immunoprecipitation/immunoblot experiments. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. TrueBlot? IP and WB Kit for DYKDDDDK (FLAG?) Epitope Tag Components: 1. Mouse IgG TrueBlot ULTRA: 50 μL 18-8817-31; 2. TrueBlot Enhancer Solution: 25 mL; 3. TrueBlot Blocker: 10 g; 4. TrueBlot Assay Buffer: 30 mL 20X; 5. TrueBlot Substrate A: 12.5 mL; 6. TrueBlot Substrate B: 12.5 mL; 7. Anti-Mouse Ig IP Beads: 2.5 mL Binds 0.4 mg Ig/mL beads; 8. Antibody for the detection of FLAG? conjugated proteins (MOUSE) Monoclonal Antibody: 100 μg; 9. Western Blot Incubation Tray. Special Notes: Upon initial use of the IP beads, we recommend that the vial be inverted several times to get the beads into suspension. We recommend using a large bore pipet to pipet up the liquid for use. For storage of the opened vial of beads, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user.
- 產(chǎn)品特點(diǎn)
- 該TrueBlot? Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG?) Epitope Tag - 88-8887-383的詳細(xì)信息查看Rockland提供的產(chǎn)品說明書。
- 保存建議
- Store Kit at 2-8 °C, except Mouse TrueBlot? ULTRA and Antibody for the detection of FLAG? conjugated proteins (MOUSE) Monoclonal Antibody, which should be stored at -20 °C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.
- 其他
- Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國費(fèi)城郊區(qū)的農(nóng)場保有近20種實(shí)驗(yàn)動(dòng)物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。
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