- 中文名稱
Androstenedione BioAssay ELISA Kit
- 英文名字
- Androstenedione BioAssay ELISA Kit
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- A2292-20
- 產(chǎn)品報(bào)價(jià)
- ￥1/1Kit
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場營銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- Androstenedione is synthesized in the adrenal gland. It is the primary precursor of testosterone for women. Therefore, in women, androstenedione contributes significantly to the level of androgenic activity, while in men this contribution is nil. Quantitation of androstenedione may indicate the androgenic activity in women.Principle of the Assay:This is an ELISA for the quantitative analysis of Androstenedione levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the Androstenedione in the sample for a limited number of binding sites on the antibody coated plate.The sample or standard solution is first added to the microplate. Next, the diluted enzymeconjugate is added and the mixture is shaken and incubated at room temperature for onehour. During the incubation, competition for binding sites is taking place. The plate is thenwashed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate, which generates an optimal color after 30 minutes.Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of Androstenedione in the sample or standard. For example, the absence of Androstenedione in the sample will result in a bright blue color, whereas the presence of Androstenedione will result in decreased or no color development.Kit Components:A2292-20A: ELISA Buffer, 1x30ml.To be used to dilute enzyme conjugate and Androstenedione standards.A2292-20B: Wash Buffer (10x), 1x20ml.To be diluted 10x with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation.A2292-20C: TMB Substrate, 1x20ml.Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus hydrogen peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed. Keep substrate refrigerated. It is light-sensitive.A2292-20D: Extraction Buffer (5x), 1x30ml.To be diluted 5x with deionized water. This is used for diluting extracted and non-extracted samples.A2292-20E: Androstenedione (HRP), 1x150ul.A2292-20F: Androstenedione Standard, 1x100ul. Androstenedione standard with a concentration of 1mg/ml.A2292-20G: Microtiter Strips, 1x96wellsA 96-well microtiter plate coated with rabbit anti- Androstenedione in each well. The plate is ready for use as is. Do not wash.Storage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?
- 產(chǎn)品特點(diǎn)
- 針對(duì)Androstenedione BioAssay ELISA Kit該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到Androstenedione BioAssay ELISA Kit產(chǎn)品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
-
- 注意
-
該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
-
