- 中文名稱
ANGPTL3, Human, BioAssay ELISA Kit (Angiopoietin-related Protein 3, Angiopoietin-5, ANG-5, Angiopoietin-like Protein 3, ANGPT5, UNQ153/PRO179)
- 英文名字
- ANGPTL3, Human, BioAssay ELISA Kit (Angiopoietin-related Protein 3, Angiopoietin-5, ANG-5, Angiopoietin-like Protein 3, ANGPT5, UNQ153/PRO179)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號
- A2293-48Q
- 產(chǎn)品報價
- ￥1/100Tests
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
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- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊、技術(shù)支持團(tuán)隊、市場營銷團(tuán)隊、進(jìn)出口報關(guān)團(tuán)隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- The angiopoietins are a family of growth factors that are specific for vascular endothelium. The full-length cDNA encoding angiopoietin-like protein 3 (ANGPTL3) from a human fetal liver/spleen cDNA library has 460aa and the characteristic structure of angiopoietins: a signal peptide, an extended helical domain predicted to form dimeric or trimeric coiled-coils, a short linker peptide, and a globular fibrinogen-like domain (FLD). Human ANGPTL3 shares 76% aa sequence identity with mouse Angptl3. Northern blot analysis of human tissues showed a preferential expression of 4 ANGPTL3 transcripts being 4.5, 3.0, 2.8, and 1.7kb in liver. ANGPTL3 can induce angiogenesis in the rat corneal assay. The FLD alone was sufficient to induce endothelial cell adhesion and in vivo angiogenesis. Microarray analysis showed that mouse hematopoietic stem cell (HSC)-supportive fetal liver CD3-positive cells expressed Angptl2 and Angptl3. The ANGPTL3 ELISA Kit is to be used for the in vitro quantitative determination of human ANGPTL3 in biological fluids. This assay is a sandwich ELISA for quantitative determination of human ANGPTL3 in biological fluids. A monoclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a purified polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples.Sample Type:Cell and tissue culture supernatants, urine, plasma and serum.Intended Use:For the in vitro quantitative determination of human ANGPTL3 in biological fluids.Sensitivity:75pg/ml Range:0.156-10ng/ml ANGPTL3/mlTest Principle:This assay is a sandwich ELISA for quantitative determination of human ANGPTL3 in biological fluids. A monoclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a purified polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples.Kit Components:A2293-48Q1: Microtiter Strips, 1x96wellsA2293-48Q2: Wash Buffer, 10X, 2x30mlA2293-48Q3: ELISA Buffer, 10X, 2x30mlA2293-48Q4: Detection Antibody, 1x50ulA2293-48Q5: Streptavidin (HRP), 100X, 1x150ulA2293-48Q5: Streptavidin (HRP), 100X, 1x150ul*A2293-48Q6: ANGPTL3 Standard (lyophilized), 1x1 vialA2293-48Q8: Substrate Solution (TMB), 1x12mlA2293-48Q9: Stop Solution (Phosphoric Acid), 1x12mlStorage and Stability:Store *A2293-48Q6 at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產(chǎn)品特點(diǎn)
- 針對ANGPTL3, Human, BioAssay ELISA Kit (Angiopoietin-related Protein 3, Angiopoietin-5, ANG-5, Angiopoietin-like Protein 3, ANGPT5, UNQ153/PRO179)該產(chǎn)品的特點(diǎn)優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到ANGPTL3, Human, BioAssay ELISA Kit (Angiopoietin-related Protein 3, Angiopoietin-5, ANG-5, Angiopoietin-like Protein 3, ANGPT5, UNQ153/PRO179)產(chǎn)品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。
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