FLAG-tag 多克隆抗體-FLAG-tag Polyclonal Antibody代理/參數(shù)/廠家-標簽抗體-艾美捷科技有限公司

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背景資料: The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
蛋白別名為：DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope;

應用類型: WB，ELISA
補充：最優(yōu)的抗體稀釋比例需要基于客戶實驗進行優(yōu)化.建議的起始稀釋比例如下: WB: 1:1000-1:3000, ELISA: 1:20000. Not yet tested in other applications.

保存建議: 廠家推薦藍冰運輸.在發(fā)貨后能在-20℃保存1年.為最大限度的避免損失，請在打開管蓋之前融化抗體并離心.我們建議使用前分裝以避免反復凍融.
本產(chǎn)品為液體形式,溶解于PBS緩沖液中，含50%甘油，0.5%BSA和0.02%NaN3.

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