MBP-Tag 多克隆抗體-MBP-Tag Polyclonal Antibody代理/參數(shù)/廠家-標(biāo)簽抗體-艾美捷科技有限公司

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背景資料: Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose.
蛋白別名為：ECK4026; JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; MMBP; Periplasmic maltose binding protein;

應(yīng)用類型: WB
補(bǔ)充：最優(yōu)的抗體稀釋比例需要基于客戶實(shí)驗(yàn)進(jìn)行優(yōu)化.建議的起始稀釋比例如下: WB: 1:5000.

保存建議: 廠家推薦藍(lán)冰運(yùn)輸.在發(fā)貨后能在-20℃保存1年.為最大限度的避免損失，請(qǐng)?jiān)诖蜷_(kāi)管蓋之前融化抗體并離心.我們建議使用前分裝以避免反復(fù)凍融.
本產(chǎn)品為液體形式,溶解于PBS緩沖液（pH 7.4）中, 含有0.02%NaN3的防腐劑和50%甘油.

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