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中文名稱(chēng)

IGFBP-4，完整 ELISA

英文名字
IGFBP-4, Intact ELISA
供應(yīng)商
Ansh Labs
產(chǎn)品貨號(hào)
AL-128
產(chǎn)品報(bào)價(jià)
￥詢(xún)價(jià)/96wellmicrotiter
產(chǎn)品說(shuō)明書(shū)
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產(chǎn)品新聞
背景資料
Insulin-like growth factor-binding protein-4 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The cDNA for human IGFBP-4 encodes a 258-residue protein that is processed, by removal of the signal sequence, to a mature protein of 237 residues (25.6 kDa) with a single asparagine-linked glycosylation site (1). Although various cell types when in culture secrete both glycosylated (28-29 kDa) and nonglycosylated (24-25 kDa) forms of IGFBP-4, the nonglycosylated is typically the most abundant in normal human blood (2, 3).
n
nIGFBP-4 is unique among the six IGFBPs in having two extra cysteine residues in the variable L-domain and may be responsible for the distinctive biological functions of IGFBP-4 (4). Although the exact functional role for serum IGFBP-4 is not absolutely clear, in vitro studies have shown that IGFBP-4 inhibits IGF activity in bone cells and other cell types. IGFBP-4 has been reported to inhibit IGF-I- and IGF-II-induced cell proliferation of embryonic chick calvaria cells and MC3T3-E1 mouse osteoblasts (5, 6), IGF-I- and IGF-II stimulated DNA synthesis in a variety of cell types (3).
n
nProteolysis is a major regulatory mechanism of IGFBP-4 functions. An IGF-dependent IGFBP-4-specific protease was first reported in the media conditioned by both human and sheep dermal fibroblasts. This protease was later identified as pregnancy-associated plasma protein-A (PAPP-A). It was shown that recombinant PAPP-A is an active protease able to cleave IGFBP-4 at a single site, between M135/K136. IGFBP-4 cleavage by PAPP-A is possible only in case when IGFBP is complexed with IGF. PAPP-A also cleaves IGFBP-5 between S143/K144, but in this case the presence of IGF is not required.
n
nSeveral studies have shown that concentration of PAPP-A in blood of patients with acute coronary syndrome (ACS) is higher than in blood of patients with stable coronary artery disease or control subjects. PAPP-A has been suggested as a marker of cardiovascular diseases associated with coronary artery blood clotting, such as unstable angina and myocardial infarction (MI) (7-14). It was hypothesized that in atherosclerotic plaques PAPP-A expressed by activated smooth muscles cells could function as an active enzyme cleaving IGFBP-4 complexed with IGF, thus enhancing IGF bioavailability. The IGF system might contribute to the atherosclerotic plaque development, destabilization, and rupture leading to acute coronary events (15). It was shown that IGFBP-4 is expressed by different cells of tumor origin, such as lung adenocarcinoma, non-small-cell lung cancer, breast cancer, colon carcinoma, follicular thyroid carcinoma, gastric cancer, glioma, hepatoma, myeloma, neuroblastoma, osteosarcoma and prostate cancer. In vitro and in vivo studies suggest that IGFBP-4 plays an important role in the growth regulation of a variety of tumors, possibly by inhibiting autocrine IGF actions. Regulation of IGF bioavailability may play crucial role in tumor growth and development (13).
n
nThe measurements of IGFBP-4 along with PAPP-A enzyme activity could be of higher clinical value than just PAPP-A measurements as PAPP-A concentration in blood is affected by heparin injections. The concentration of PAPP-A, total IGFBP-4 and intact IGFBP-4 in biological fluid can be measured accurately using immunoassay methods (picoPAPP-A ELISA; AL-101, Total IGFBP-4 ELISA; AL-126 and Intact IGFBP-4 ELISA; AL-128, respectively). The ratio of total to Intact IGFBP-4 concentration measured in individual subject over time will help normalizes the IGFBP-4 variability between subjects and also increase the detection rate of increased PAPP-A activity in MI subjects. The immunoassay methods designed for the measurement of total and Intact IGFBP-4 in patient samples could be of practical value for the diagnosis or prediction of various pathologies including ACS and cancer.
n
nReferences:
n1. La Tour D, Mohan S, Linkhart T A, Baylink D J, Strong D D. Inhibitory insulin-like growth factor binding protein: cloning, complete sequence, and physiologic regulation. Mol Endocrinol. 1990; 4:1806-1814.
n2. Baxter R C, Martin J L. Binding proteins for the insulin-like growth factors: structure, regulation and function. Prog in Growth Factor Res. 1989; 1:49-68.
n3. Rechler M M., Insulin-like growth factor binding proteins. Vitam Horm. 1993; pp. 471-114.
n4. Zhou R, Diehl D, Hoeflich A, Lahm H, Wolf E., IGF-binding protein-4: biochemical characteristics and functional consequences. Journal of Endocrinology 2003; 178: 177-193.
n5. Mohan S, Bautista C, Wergedal J, Baylink D J. Isolation of an inhibitory insulin-like growth factor (IGF) binding protein from bone cell conditioned medium: a potential local regulator of IGF action. Proc Nat Acad Sci USA. 1989; 86:8338-8342.
n6. Mohan S, Nakao Y, Honda Y, et al., Studies on the molecular mechanisms by which insulin-like growth factor (IGF) binding protein-4 (IGFBP-4) and IGFBP-5 modulate IGF actions in bone cells. J Biol Chem. 1995; 270:20424-20431.
n7. Qin Q, Wittfooth S, Pettersson K. Measurement and clinical significance of circulating PAPP-A in ACS patients. Clin Chim Acta. 2007;380:59-67.
n8. Iversen KK, Teisner AS, Teisner B, et al. Pregnancy Associated Plasma Protein A, a Novel, Quick, and Sensitive marker in ST-Elevation Myocardial Infarction. Am J Cardiol. 2008;101:1389-1394.
n9. Lund J, Qin Q, Ilva T, et al. Pregnancy-associated plasma protein A: A biomarker in acute ST-elevation myocardial infarction (STEMI). Annals of Medicine. 2006;38:221-228.
n10. Elesber AA, Lerman A, et al. Pregnancy associated plasma protein-A and risk stratification of patients presenting with chest pain in the emergency department. Int J Cardiol. 2007;117:365-369.
n11. Heeschen C, Dimmeler S, et al. Pregnancy-Associated Plasma Protein-A Levels in Patients With Acute Coronary Syndromes. JACC. 2005;45(2):229-237.
n12. Lund J, Qin Q, Ilva T, et al. Circulating Pregnancy-Associated Plasma Protein A Predeicts Outcome in Patients With Acute Coronary Syndrome but No Troponin I Elevation. Circulation. 2003;108:1924-1926.
n13. Bayes-Genis A, Conover CA, et al. Pregnancy-Associated Plasma Protein A as a Marker of Acute Coronary Syndromes. NEJM. 2001;345(14):1022-1029.
n14. Bonaca M P, et.al Prospective Evaluation of Pregnancy-Associated Plasma Protein-A and Outcomes in Patients With Acute Coronary Syndromes. JACC. 2012;60(4):332-338.
n15. Libby P, What happens inside an atherosclerotic plaque? International Congress Series, 2004; 1262 253-256
n16. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
n17. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 - Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.
n18. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038
產(chǎn)品描述
The Intact IGFBP-4 enzyme linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of IGFBP-4 in human serum and other biological fluids.
產(chǎn)品特點(diǎn)
保存建議
Store at 2 to 8°C until expiration date.
其他
Ansh labs是開(kāi)發(fā)和生產(chǎn)免疫檢測(cè)試劑盒和定制生物技術(shù)檢測(cè)產(chǎn)領(lǐng)創(chuàng)者，總部位于美國(guó)休斯敦，公司產(chǎn)品遍及全球80多個(gè)國(guó)家。公司于2011年成立，核心團(tuán)隊(duì)來(lái)自于Diagnostic system laboratory，專(zhuān)注于女性健康和激素檢測(cè)領(lǐng)域近40年，擁有全球領(lǐng)先的核心技術(shù)， 以及多年經(jīng)驗(yàn)生物技術(shù)研發(fā)經(jīng)驗(yàn)的科學(xué)家團(tuán)隊(duì)，產(chǎn)品覆蓋生殖健康，高危妊娠，腫瘤，糖尿病等多個(gè)疾病領(lǐng)域，圍繞最新的免疫分析技術(shù)，多種蛋白和單克隆抗體，如AMH，BMP-15, Follistatin, GDF-9, IGF-I, IGF-II, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, Inhibin Alpha, Inhibin ?A, Inhibin ?B, MBP, PAPP-A, PAPP-A2 and Vitamin D.等幾十余種試劑產(chǎn)品，也同時(shí)涵蓋小鼠，大鼠，狗，羊，牛等靈長(zhǎng)類(lèi)多個(gè)物種的獨(dú)特的物種特異性的試劑。 Ansh labs 坐落于美國(guó)德克薩斯州，是目前世界上唯一能提供TGF-β超家族所有成員的檢測(cè)公司，也是最早專(zhuān)注激素類(lèi)產(chǎn)品研究和新型免疫檢測(cè)技術(shù)的先驅(qū)者。優(yōu)勢(shì)產(chǎn)品涉及TGF-β super family、AMH/抑制素B(human/animal)、full panel IGFs、以及最完整的胰高血糖素系列。
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