FLAG-tag is a tag that can be added to a protein sequence motif DYKXXD. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
應(yīng)用類型
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
免疫原
KLH-conjugated DYKDDDDK (FLAG) synthetic peptide
來(lái)源宿主
小鼠
反應(yīng)性
N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins