The reactivity of the antiserum is directed to the subclass IgG2a. It reacts with IgG2a of the allelic types Igh-1a and Igh-1b, which include BALB/C, C57Bl, CBA/J, SJL/J and SM/J. hen used for the identification of IgG2a in other mouse strains the reaction may be weaker. The immunoconjugate contains antibodies to iso and allospecific determinants. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
應(yīng)用類(lèi)型
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
免疫原
Pools of purified homogenous IgG2a isolated from pooled mouse sera belonging to the allelic types Igh- 1a and Igh-1b, Freund's complete adjuvant is used in the first step of the immunization procedure,
來(lái)源宿主
山羊
反應(yīng)性
小鼠 IgG2a
保存建議
The lyophilized conjugate is shipped at ambient temperature and may be stored at 4