中文名稱(chēng)

BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500

英文名字

BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500

供應(yīng)商

Rockland

產(chǎn)品貨號(hào)

B501-0500

產(chǎn)品報(bào)價(jià)

￥詢(xún)價(jià)/500g

產(chǎn)品說(shuō)明書(shū)

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購(gòu)買(mǎi)方式

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產(chǎn)品新聞

背景資料

Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below).? These products are intended to block non-specific binding of proteins in various immunological assays.? Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to the addition of primary antibody.

產(chǎn)品描述

Use Rockland's BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk.?? Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available.? BLOTTO A is a convenient general purpose blocking agent.? BLOTTO B is recommended for blocking when phospho-specific antibodies are used.? BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025).? Users should consider the addition of protease inhibitors and phosphatase inhibitors when required.? Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity.

產(chǎn)品形式

Lyophilized

保存建議

Store container at room temperature prior to opening. After reconstitution, use blocking buffers immediately. Dilute solutions may be stored at 4° C for up to four (4) days. Solutions containing BLOTTO may be frozen.

其他

Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國(guó)費(fèi)城郊區(qū)的農(nóng)場(chǎng)保有近20種實(shí)驗(yàn)動(dòng)物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號(hào)蛋白、酶類(lèi)、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識(shí)別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。

注意

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