ClickTech DNA FISH 套件 488-ClickTech DNA FISH Kit 488代理/參數(shù)/廠家-ELISA試劑盒-艾美捷科技有限公司

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中文名稱: ClickTech DNA FISH 套件 488

英文名字: ClickTech DNA FISH Kit 488

供應商: Baseclick

產品貨號: BCK-DNA-FISH-488

產品報價: ￥詢價/1kit

產品說明書: 點擊查看

購買方式: 銀行轉賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868

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背景資料: ClickTech DNA FISH 套件 488(ClickTech DNA FISH Kit 488)背景資料,請查看該產品的使用說明書

產品描述: Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that enables the detection and localization of specific DNA or RNA sequences within cells/tissues. DNA FISH is a very important tool not only for basic research, but also for clinical applications such as cytogenetic screening for diagnosis and prognosis of cancer (Oliveira and French 2005) and genetic syndromes (Volpi and Bridger 2008a), including prenatal screening. The principle of DNA FISH is to hybridize small single stranded DNA molecules (probes) to complementary sequences on chromosome spreads or in nuclei of whole cells (target), which are immobilized on glass slides. The probes are labeled directly or indirectly using fluorescent or radioactive nucleotides or through fluorescent antibodies (Volpi and Bridger 2008b). After labeling, FISH probes and targets are thermally denatured and hybridized.The standard FISH technique, however, often suffers from limitations, namely poor signal to background noise ratio due to unspecific binding of probes to only partially complementary sequences or other cellular and tissue structures. In addition, while even routine in situ detection highly benefits from densely labelled, sensitive probes, the labelling rate of FISH probes generated by traditional methods is typically low.baseclick’s ClickTech DNA FISH Kit overcomes these limitations and provides all reagents required for the generation of highly labelled FISH probes via an easy-to-handle method based on click chemistry. During enzymatic PCR synthesis, unmodified (dNTPs) and alkyne modified- (EdUTP) nucleotides are used by our baseclick Ethynyl Polymerase, producing a clickable PCR product (DNA FISH probe). By using click reagents (incl. in the kit) and a clickable dye (incl. in the kit) of your choice (green 488, yellow 555, orange 594 or red 647) you obtain DNA fluorescent labeled DNA FISH probes.

產品特點: ClickTech DNA FISH 套件 488(ClickTech DNA FISH Kit 488)產品特點,請查看該產品的使用說明書

保存建議: -20 °C

其他: Baseclick于2008成立于2008年德國慕尼黑，BaseClick的核心競爭力源于其專有的銅催化疊氮-炔環(huán)加成（CuAAC）點擊化學技術。該技術由2001年諾貝爾化學獎得主Barry Sharpless教授發(fā)明，BaseClick擁有在核酸領域應用該技術的全球獨家許可。相比傳統(tǒng)方法，點擊化學具有反應迅速、條件溫和、產率高、選擇性強等顯著優(yōu)勢，可實現(xiàn)從小分子寡核苷酸到大基因片段的高效、精準功能化修飾。目前的應用領域包括：核酸標記技術、Edu細胞增殖試驗，PCR標記試劑盒，F(xiàn)ISH探針，（高度）修飾的寡核苷酸等等，Baseclick跟Thermo Fisher，Roche，Merck，abcam，BD Bioscience等諸多知名品牌建立了合作關系。