- 中文名稱
ClickTech DNA FISH 套件 555
- 英文名字
- ClickTech DNA FISH Kit 555
- 供應(yīng)商
- Baseclick
- 產(chǎn)品貨號(hào)
- BCK-DNA-FISH-555
- 產(chǎn)品報(bào)價(jià)
- ￥詢價(jià)/1kit
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購(gòu)買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- ClickTech DNA FISH 套件 555(ClickTech DNA FISH Kit 555)背景資料,請(qǐng)查看該產(chǎn)品的使用說明書
- 產(chǎn)品描述
- Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that enables the detection and localization of specific DNA or RNA sequences within cells/tissues. DNA FISH is a very important tool not only for basic research, but also for clinical applications such as cytogenetic screening for diagnosis and prognosis of cancer (Oliveira and French 2005) and genetic syndromes (Volpi and Bridger 2008a), including prenatal screening. The principle of DNA FISH is to hybridize small single stranded DNA molecules (probes) to complementary sequences on chromosome spreads or in nuclei of whole cells (target), which are immobilized on glass slides. The probes are labeled directly or indirectly using fluorescent or radioactive nucleotides or through fluorescent antibodies (Volpi and Bridger 2008b). After labeling, FISH probes and targets are thermally denatured and hybridized.The standard FISH technique, however, often suffers from limitations, namely poor signal to background noise ratio due to unspecific binding of probes to only partially complementary sequences or other cellular and tissue structures. In addition, while even routine in situ detection highly benefits from densely labelled, sensitive probes, the labelling rate of FISH probes generated by traditional methods is typically low.baseclick’s ClickTech DNA FISH Kit overcomes these limitations and provides all reagents required for the generation of highly labelled FISH probes via an easy-to-handle method based on click chemistry. During enzymatic PCR synthesis, unmodified (dNTPs) and alkyne modified- (EdUTP) nucleotides are used by our baseclick Ethynyl Polymerase, producing a clickable PCR product (DNA FISH probe). By using click reagents (incl. in the kit) and a clickable dye (incl. in the kit) of your choice (green 488, yellow 555, orange 594 or red 647) you obtain DNA fluorescent labeled DNA FISH probes.
- 產(chǎn)品特點(diǎn)
- ClickTech DNA FISH 套件 555(ClickTech DNA FISH Kit 555)產(chǎn)品特點(diǎn),請(qǐng)查看該產(chǎn)品的使用說明書
- 保存建議
- -20 °C
- 其他
- Baseclick于2008成立于2008年德國(guó)慕尼黑，BaseClick的核心競(jìng)爭(zhēng)力源于其專有的銅催化疊氮-炔環(huán)加成（CuAAC）點(diǎn)擊化學(xué)技術(shù)。該技術(shù)由2001年諾貝爾化學(xué)獎(jiǎng)得主Barry Sharpless教授發(fā)明，BaseClick擁有在核酸領(lǐng)域應(yīng)用該技術(shù)的全球獨(dú)家許可。相比傳統(tǒng)方法，點(diǎn)擊化學(xué)具有反應(yīng)迅速、條件溫和、產(chǎn)率高、選擇性強(qiáng)等顯著優(yōu)勢(shì)，可實(shí)現(xiàn)從小分子寡核苷酸到大基因片段的高效、精準(zhǔn)功能化修飾。目前的應(yīng)用領(lǐng)域包括：核酸標(biāo)記技術(shù)、Edu細(xì)胞增殖試驗(yàn)，PCR標(biāo)記試劑盒，F(xiàn)ISH探針，（高度）修飾的寡核苷酸等等，Baseclick跟Thermo Fisher，Roche，Merck，abcam，BD Bioscience等諸多知名品牌建立了合作關(guān)系。
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- 注意
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該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
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