FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity).
產(chǎn)品描述
The rat BDNF Kit is a sandwich ELISA. The capture antibody is a monoclonal BDNF antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known BDNF concentration are added to these wells and allowed to complex with the bound BNDF antibody. A biotinylated BDNF antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BDNF present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat BDNF in samples such as sera, plasma, cell lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
產(chǎn)品特點
靈敏度:31.2 pg/mL - 2000 pg/mL。此外該ELIAS試劑盒包含:2 x 96孔預(yù)包被條孔板(可拆卸), 蛋白質(zhì)標準品,檢測試劑,底物緩沖液,以及詳細的實驗方案。