Intercellular adhesion molecule 1 (ICAM1) is a single-pass type 1 membrane glycoprotein that belongs to the ICAM family. ICAM1 is typically expressed on endothelial cells and cells of the immune system.
產品描述
The rat ICAM-1 Kit is a sandwich ELISA. The capture antibody is a mouse monoclonal ICAM-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known ICAM-1 concentration are added to these wells and allowed to complex with the bound ICAM-1 antibody. A biotinylated rat ICAM-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ICAM-1 present in the samples. The purpose of this kit is the in-vitro quantitative detection of rat ICAM-1/CD54 in cell culture supernatants, cell lysates, serum, and heparin or EDTA treated plasma samples only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
產品特點
靈敏度:31.2 pg/mL - 2000 pg/mL。此外該ELIAS試劑盒包含:1 x 96孔預包被條孔板(可拆卸), 蛋白質標準品,檢測試劑,底物緩沖液,以及詳細的實驗方案。