The mouse Interleukin 12 (p70) Kit is a sandwich ELISA. The capture antibody is a monoclonal specific for mouse IL-12(p70) antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and recombinant standards of known IL-12(p70) concentration are added to these wells and allowed to complex with the bound IL-12(p70) antibody. A biotinylated polyclonal IL-12(p70) specific antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-12(p70) present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-12(p70) in samples of cell culture supernatants and serum . This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.
產(chǎn)品特點(diǎn)
靈敏度:15.6 pg/mL - 1000 pg/mL。此外該ELIAS試劑盒包含:2 x 96孔預(yù)包被條孔板(可拆卸), 蛋白質(zhì)標(biāo)準(zhǔn)品,檢測試劑,底物緩沖液,以及詳細(xì)的實(shí)驗(yàn)方案。