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ELISA試劑盒
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中文名稱

NGF快速ELISA試劑盒:小鼠(2個(gè)板)

英文名字
NGF Rapid ELISA Kit: Mouse (2 Plates)
供應(yīng)商
Biosensis
產(chǎn)品貨號(hào)
BEK-2213-2P
產(chǎn)品報(bào)價(jià)
¥詢價(jià)/2Plate
產(chǎn)品說明書
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產(chǎn)品新聞
背景資料
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
產(chǎn)品描述
The Biosensis NGF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse NGF in less than 3 hours in cell culture supernatants and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.
This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards.
This NGF ELISA kit is designed to measure mouse NGF and thus employs a mouse NGF standard. The mouse NGF standard supplied has been purified from mouse submaxillary glands according to published procedures. The calibrator standard reflects the native state of mouse NGF protein and has been chosen to give most accurate quantification of natural NGF protein levels in mouse samples.
Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! This ELISA kit preferentially detects the mature form of NGF. Cross- reaction of full-length mouse proNGF was < 0.1% when assayed at 25 ng/mL in assay buffer, and not detectable when assayed across the mature NGF calibration range.
This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
產(chǎn)品特點(diǎn)
靈敏度:3.9 - 250 pg/mL。此外該ELIAS試劑盒包含:2 x 96孔預(yù)包被條孔板(可拆卸), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
保存建議
廠家推薦冰袋運(yùn)輸。廠家推薦試劑盒保存于4 °C
其他
Biosensis專注于神經(jīng)科學(xué)領(lǐng)域的抗體和試劑的開發(fā),特別是神經(jīng)營養(yǎng)因子和神經(jīng)營養(yǎng)因子受體。 近30年來,Biosensis一直是該領(lǐng)域的全球領(lǐng)航者和OEM供應(yīng)商。除神經(jīng)營養(yǎng)因子,我們的神經(jīng)科學(xué)產(chǎn)品組合還被廣泛用于神經(jīng)退行性疾病、神經(jīng)發(fā)育和神經(jīng)代謝的研究。重點(diǎn)研究領(lǐng)域包括阿爾茨海默氏癥(AD)、帕金森氏癥(PD)和肌萎縮側(cè)索硬化(ALS)疾病,以及自噬和代謝應(yīng)激障礙,包括肥胖、代謝綜合征的研究、神經(jīng)免疫學(xué)和炎癥。Biosensis的產(chǎn)品系列不僅包括持續(xù)增長的神經(jīng)科學(xué)研究抗體系列,還包括200多種定量研究ELISAs試劑盒(1板或2板,自由選擇),組織染色(退化神經(jīng)元染色試劑盒、病理髓鞘染色試劑盒、淀粉樣斑塊染色試劑等)和細(xì)胞可視化試劑(染色)以及針對神經(jīng)科學(xué)和細(xì)胞疾病研究的純化的蛋白質(zhì)。我們的抗體和試劑已被廣泛應(yīng)用于各種技術(shù),包括蛋白質(zhì)印跡,免疫組織化學(xué),流式分析,共聚焦顯微鏡實(shí)時(shí)成像,生物抑制和細(xì)胞培養(yǎng)以及克隆。
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