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ELISA試劑盒
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中文名稱

Neurotrophin 3(NT3)快速ELISA試劑盒:人,大鼠和小鼠(1個板)

英文名字
Neurotrophin 3 (NT3) Rapid ELISA Kit: Human, Rat and Mouse (1 Plate)
供應(yīng)商
Biosensis
產(chǎn)品貨號
BEK-2221-1P
產(chǎn)品報價
¥詢價/1Plate
產(chǎn)品說明書
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購買方式
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產(chǎn)品新聞
背景資料
NT3 is understood to be important in promote the survival of visceral and proprioceptive sensory neurons. It is a secreted protein that belongs to the NGF-beta family and is found in brain and peripheral tissues.
產(chǎn)品描述
The Biosensis NT3 RapidTM enzyme-linked immune-sorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT3 in less than 4 hours in cell culture supernatants and human plasma (EDTA and citrate) only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human plasma. Accurate quantification of NT3 in human plasma requires a heterophilic antibody (HA) blocker which can be purchased separately (BL-004-500).
This ELISA kit consists of a pre-coated polyclonal anti-NT3 capture antibody, a biotinylated monoclonal anti-NT3 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT3 present in samples and protein standards.
This NT3 RapidTM ELISA kit employs a recombinant human NT3 standard. NT3 is highly conserved and nearly identical in many species. The assay's capture and detection antibodies detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 RapidELISA will also react with those species and possibly more because of the conversation of the immunogen used.
The antibodies used in this kit bind to epitopes within the mature domain of NT3. Thus, this ELISA kit may detect the full-length pro-form of NT3.
This kit has not been tested for other applications. Sufficient amount of NT3 standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
產(chǎn)品特點
該ELIAS試劑盒包含:1 x 96孔預(yù)包被條孔板(可拆卸), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
保存建議
廠家推薦冰袋運輸。廠家推薦試劑盒保存于2-8 °C.
其他
Biosensis專注于神經(jīng)科學(xué)領(lǐng)域的抗體和試劑的開發(fā),特別是神經(jīng)營養(yǎng)因子和神經(jīng)營養(yǎng)因子受體。 近30年來,Biosensis一直是該領(lǐng)域的全球領(lǐng)航者和OEM供應(yīng)商。除神經(jīng)營養(yǎng)因子,我們的神經(jīng)科學(xué)產(chǎn)品組合還被廣泛用于神經(jīng)退行性疾病、神經(jīng)發(fā)育和神經(jīng)代謝的研究。重點研究領(lǐng)域包括阿爾茨海默氏癥(AD)、帕金森氏癥(PD)和肌萎縮側(cè)索硬化(ALS)疾病,以及自噬和代謝應(yīng)激障礙,包括肥胖、代謝綜合征的研究、神經(jīng)免疫學(xué)和炎癥。Biosensis的產(chǎn)品系列不僅包括持續(xù)增長的神經(jīng)科學(xué)研究抗體系列,還包括200多種定量研究ELISAs試劑盒(1板或2板,自由選擇),組織染色(退化神經(jīng)元染色試劑盒、病理髓鞘染色試劑盒、淀粉樣斑塊染色試劑等)和細胞可視化試劑(染色)以及針對神經(jīng)科學(xué)和細胞疾病研究的純化的蛋白質(zhì)。我們的抗體和試劑已被廣泛應(yīng)用于各種技術(shù),包括蛋白質(zhì)印跡,免疫組織化學(xué),流式分析,共聚焦顯微鏡實時成像,生物抑制和細胞培養(yǎng)以及克隆。
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