UltraScence Pico Ultra Western Substrate是一種基于魯米諾的增強化學發(fā)光底物,它敏感且兼容辣根過氧化物酶(HRP)偶聯(lián)二抗體進行免疫印跡。UltraScence Pico Ultra Western Substrate優(yōu)異的靈敏度和長信號持續(xù)時間使抗原的低圖像到中飛圖檢測成為可能。此外,它的長化學發(fā)光信號持續(xù)時間使數(shù)字和基于薄膜的成像不損失任何信號成為可能。建議適當?shù)囊豢购投瓜♂屢垣@得最佳的信號強度和持續(xù)時間。—無需優(yōu)化。切換到其他品牌的UltraScence Pico Ultra Western襯底,如Pierce和GE Healthcare,不需要優(yōu)化或協(xié)議更改。—靈敏度高,化學發(fā)光持續(xù)時間長。UltraScence Pico Ultra Western Substrate能夠在單一暴露后,對同一免疫印跡上的蛋白質(zhì)進行精確的低圖像圖到中飛圖檢測。—優(yōu)化使用PVDF和硝化纖維素膜。—與Western Blotting標記兼容。—為基于薄膜和ccd的成像優(yōu)化。
產(chǎn)品描述
The UltraScence Pico Ultra Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to mid femtogram detection of antigen is enabled by UltraScence Pico Ultra Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration. --No optimization required. Switching to the UltraScence Pico Ultra Western Substrate from other brands, such as Pierce and GE Healthcare, does not require optimization or protocol changes. --High degree of sensitivity and enhanced chemiluminescence duration. UltraScence Pico Ultra Western Substrate enables an accurate low picogram to mid femtogram detection of protein on the same immunoblot after a single exposure. --Optimized for use with PVDF and nitrocellulose membranes. --Compatible with Western Blotting Markers. --Optimized for film- and CCD-based imaging.