UltraScence Femto Plus Western Substrate,作為一種基于發(fā)光氨的增強(qiáng)化學(xué)發(fā)光基底,與辣根過氧化物酶(HRP)結(jié)合的二次抗體。UltraScence Femto Plus Western襯底是為檢測目標(biāo)而設(shè)計的蛋白質(zhì)的數(shù)量太小,在典型的ECL底物中看不到。UltraScence Femto Plus Western Substrate優(yōu)異的靈敏度和長時間的檢測能力使抗原的低飛圖檢測(中飛)成為可能信號持續(xù)時間。此外,它的長化學(xué)發(fā)光信號持續(xù)時間使數(shù)字和基于薄膜的成像在沒有任何信號丟失的情況下是可能的。建議適當(dāng)?shù)囊弧⒍贵w稀釋獲得最佳信號強(qiáng)度和持續(xù)時間。此外,UltraScence Femto Plus Western的高穩(wěn)定性襯底可以在室溫下儲存2年,而不影響性能。
產(chǎn)品描述
The UltraScence Femto Plus Western Substrate, as a luminol-based enhanced chemiluminescent substrate,nis sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugatednsecondary antibodies. The UltraScence Femto Plus Western Substrate is designed for the detection of the targetnproteins in amounts that are too small to be seen with typical ECL substrates. The low femtogram detection (midzeptomole) of antigen is enabled by UltraScence Femto Plus Western Substrate’s excellent sensitivity and longnsignal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imagingnpossible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested fornattaining optimal signal intensity and duration. Moreover, the high stability of UltraScence Femto Plus WesternnSubstrate makes a 2-year- storage at room temperature possible, without any compromise on the performance